2x9d | pdb_00002x9d

Tet repressor (class D) in complex with iso-7-chlortetracycline

spinqualitylabelsall models 2x9d, resolution 2.34Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2x9d is a 1 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. This structure supersedes the now removed PDB entry 1du7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CL, ITC Related: 2xgc, 2xpw, 1bjz, 2trt, 2xpv, 2vkv, 1ork, 2xge, 1a6i, 2x6o, 1qpi, 2vke, 2xgd, 2xrl, 2xpt, 2xpu, 2xb5, 1bj0, 2xps, 1bjy, 2tct, 1du7 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [TETR4_ECOLX] TetR is the repressor of the tetracycline resistance element; its N-terminal region forms a helix-turn-helix structure and binds DNA. Binding of tetracycline to TetR reduces the repressor affinity for the tetracycline resistance gene (tetA) promoter operator sites. Publication Abstract from PubMed Tetracycline antibiotics and their degradation products appear in medically treated tissues, food, soil, and manure sludge in the environment. In the context of protein interactions with various tetracyclines we performed crystal structure analyses of the tetracycline repressor in complex with weak or noninducing tetracycline derivatives. Isotetracyclines are degradation products of tetracyclines, which occur under physiological conditions. The typical framework of the antibiotic is irreversibly broken at the BC-ring connection, leading to a modified orientation of the AB to the new C*D ring fragments. The shape of the zwitterionic AB-ring fragment is unchanged and still binds to the TetR recognition site in a manner comparable to the intact antibiotic but without typical Mg(2+) chelation. This work is an example that drug degradation products can still bind to specific targets and should be discussed in light of potential and critical side effects. Recognition of drug degradation products by target proteins: isotetracycline binding to Tet repressor.,Volkers G, Petruschka L, Hinrichs W J Med Chem. 2011 Jul 28;54(14):5108-15. Epub 2011 Jul 1. PMID:21699184 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Tetracycline repressor protein

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