2mrw | pdb_00002mrw

Solution Structure of MciZ from Bacillus subtilis

spinqualitylabelsall models 2mrw, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2mrw is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Cytoskeletal structures are dynamically remodeled with the aid of regulatory proteins. FtsZ (filamentation temperature-sensitive Z) is the bacterial homolog of tubulin that polymerizes into rings localized to cell-division sites, and the constriction of these rings drives cytokinesis. Here we investigate the mechanism by which the Bacillus subtilis cell-division inhibitor, MciZ (mother cell inhibitor of FtsZ), blocks assembly of FtsZ. The X-ray crystal structure reveals that MciZ binds to the C-terminal polymerization interface of FtsZ, the equivalent of the minus end of tubulin. Using in vivo and in vitro assays and microscopy, we show that MciZ, at substoichiometric levels to FtsZ, causes shortening of protofilaments and blocks the assembly of higher-order FtsZ structures. The findings demonstrate an unanticipated capping-based regulatory mechanism for FtsZ. FtsZ filament capping by MciZ, a developmental regulator of bacterial division.,Bisson-Filho AW, Discola KF, Castellen P, Blasios V, Martins A, Sforca ML, Garcia W, Zeri AC, Erickson HP, Dessen A, Gueiros-Filho FJ Proc Natl Acad Sci U S A. 2015 Apr 6. pii: 201414242. PMID:25848052 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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