7cne | pdb_00007cne

Crystal Structure of Sphingomyelinase C from Streptomyces griseocarneus

spinqualitylabelsall models 7cne, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7cne is a 1 chain structure with sequence from "streptomyces_griseocarneus"_benedict_et_al._1950 "streptomyces griseocarneus" benedict et al. 1950. This structure supersedes the now removed PDB entry 3wcx. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EPE, SIN Gene: smc ("Streptomyces griseocarneus" Benedict et al. 1950) Activity: Sphingomyelin phosphodiesterase, with EC number 3.1.4.12 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Sphingomyelinase C (SMC) hydrolyzes sphingomyelin to ceramide and phosphocholine. Prokaryotic SMCs share sequence homology with mammalian SMCs that have enzymatic pH optima at neutral pH. SMC from the nonpathogenic prokaryote Streptomyces griseocarneus shows notable enzymatic features such as higher optimum pH and thermostability than other prokaryotic SMCs. Determination of the three-dimensional structure of S. griseocarneus-SMC (Sg-SMC) and comparison with other SMC structures represents a promising strategy to elucidate the unique enzymatic features of Sg-SMC on a structural basis. Therefore, we determined the crystal structure of Sg-SMC at 2.0 A resolution by X-ray crystallography. Comparison of the Sg-SMC structure with three other structurally known SMCs from Listeria ivanovii, Bacillus cereus, and Staphylococcus aureus indicated that Sg-SMC is more diverse in sequence and that structural differences in the main chain between these SMCs are primarily located on the molecular surface distant from the active site. Comparison of the surface area of the four SMCs revealed that Sg-SMC has the most compact structure, which may contribute to the enhanced thermostability of Sg-SMC. Regarding the hydrogen bond network in the active site of Sg-SMC, a basic amino acid, Arg278, is involved, whereas the corresponding residue in other SMCs (Ser or Asn) does not form hydrogen bonds with metal-coordinating water molecules. Hydrogen bond formation between Arg278 and a Mg(2+) ion-coordinating water molecule may be responsible for the higher optimal pH of Sg-SMC compared to that of other SMCs. Structural basis for the high thermal stability and optimum pH of sphingomyelinase C from Streptomyces griseocarneus.,Fujisawa I, Hamana H, Tomita Y, Matsumoto Y, Murayama K, Sugimori D J Biosci Bioeng. 2020 Oct 3. pii: S1389-1723(20)30354-6. doi:, 10.1016/j.jbiosc.2020.09.005. PMID:33023861 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Sphingomyelinase

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