6tzn | pdb_00006tzn

Structure of S. pombe telomerase accessory protein Pof8 C-terminal domain

spinqualitylabelsall models 6tzn, resolution 1.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6tzn is a 1 chain structure with sequence from Fission yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NO3 Gene: pof8, SPAC17G6.17 (Fission yeast) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [POF8_SCHPO] RNA-binding protein required for assembly of the holoenzyme telomerase ribonucleoprotein (RNP) complex (PubMed:29422501, PubMed:29422503, PubMed:29422664). Specifically binds telomerase RNA ter1 and promotes assembly of ter1 with catalytic subunit trt1 (PubMed:29422501, PubMed:29422503). Telomerase is a ribonucleoprotein enzyme essential that copies new telomeric repeats onto chromosome ends and functions to maintain cell division (PubMed:29422501, PubMed:29422503, PubMed:29422664). Publication Abstract from PubMed La-related proteins 7 (LARP7) are a class of RNA chaperones that bind the 3' ends of RNA and are constitutively associated with their specific target RNAs. In metazoa, Larp7 binds to the long non-coding 7SK RNA as a core component of the 7SK RNP, a major regulator of eukaryotic transcription. In the ciliate Tetrahymena the LARP7 protein p65 is a component of telomerase, an essential ribonucleoprotein complex that maintains the telomeric DNA at eukaryotic chromosome ends. p65 is important for the ordered assembly of telomerase RNA (TER) with telomerase reverse transcriptase. Unexpectedly, Schizosaccharomyces pombe Pof8 was recently identified as a LARP7 protein and a core component of fission yeast telomerase essential for biogenesis. LARP7 proteins have a conserved N-terminal La motif and RRM1 (La module) and C-terminal RRM2 with specific RNA substrate recognition attributed to RRM2, first structurally characterized in p65 as an atypical RRM named xRRM. Here we present the X-ray crystal structure and NMR studies of S. pombe Pof8 RRM2. Sequence and structure comparison of Pof8 RRM2 to p65 and human Larp7 xRRMs reveals conserved features for RNA binding with the main variability in the length of the non-canonical helix alpha3. This study shows that Pof8 has conserved xRRM features, providing insight into TER recognition and the defining characteristics of the xRRM. Structure of S. pombe telomerase protein Pof8 C-terminal domain is an xRRM conserved among LARP7 proteins.,Basu R, Eichhorn CD, Cheng R, Peterson RD, Feigon J RNA Biol. 2020 Nov 1:1-12. doi: 10.1080/15476286.2020.1836891. PMID:33131423 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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