3j09 | pdb_00003j09

High resolution helical reconstruction of the bacterial p-type ATPase copper transporter CopA

Loading... Status: waiting for Mol* initialization Structural highlights 3j09 is a 2 chain structure with sequence from Arcfl. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: copA, pacS, AF_0473 (ARCFL) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [COPA_ARCFU] Probably involved in copper and silver export. Publication Abstract from PubMed CopA uses ATP to pump Cu(+) across cell membranes. X-ray crystallography has defined atomic structures of several related P-type ATPases. We have determined a structure of CopA at 10 A resolution by cryo-electron microscopy of a new crystal form and used computational molecular docking to study the interactions between the N-terminal metal-binding domain (NMBD) and other elements of the molecule. We found that the shorter-chain lipids used to produce these crystals are associated with movements of the cytoplasmic domains, with a novel dimer interface and with disordering of the NMBD, thus offering evidence for the transience of its interaction with the other cytoplasmic domains. Docking identified a binding site that matched the location of the NMBD in our previous structure by cryo-electron microscopy, allowing a more detailed view of its binding configuration and further support for its role in autoinhibition. The Architecture of CopA from Archeaoglobus fulgidus Studied by Cryo-Electron Microscopy and Computational Docking.,Allen GS, Wu CC, Cardozo T, Stokes DL Structure. 2011 Aug 3. PMID:21820315 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also ATPase 3D structures

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