5n6u | pdb_00005n6u

Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum.

spinqualitylabelsall models 5n6u, resolution 3.08Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5n6u is a 4 chain structure with sequence from Dictyoglomus thermophilum H-6-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.08Å Ligands: BMA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B5YAN4_DICT6 Publication Abstract from PubMed Glycoside hydrolases can be turned into thioglycoligase by mutation of the acid/base catalytic carboxylate residue. These mutants have proven valuable to generate S-glycosides, however, few examples in literature have described efficient thioglycoligase activity, and even fewer the underlying molecular mechanism. DtMan, a GH2 family beta-D-mannosidase from the thermophilic Dictyoglomus thermophilum was cloned and expressed in E. coli. The recombinant protein is highly specific for beta-D-mannosides, and exhibits efficient catalysis constants coupled to thermostability. However, seven variants bearing mutated acid/base residue could not be turned into efficient thioligases. Crystal structure of DtMan Glu425Cys mutant and molecular modeling calculations have demonstrated that unlike other GH2 thioligase reported, active site accessibility of thiol acceptor may be impaired by entrance loop rigidity. This structural feature may explain why DtMan mutants do not exhibit thioglycoligase activity. Is the acid/base catalytic residue mutation in beta-D-mannosidase DtMan from Dictyoglomus thermophilum sufficient enough to provide thioglycoligase activity?,Guillotin L, Richet N, Lafite P, Daniellou R Biochimie. 2017 Apr 3. pii: S0300-9084(17)30085-8. doi:, 10.1016/j.biochi.2017.03.020. PMID:28385558 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Mannosidase 3D structures

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