8b7o | pdb_00008b7o
X-ray structure of Auranofin-human H-chain ferritin
Structural highlights
FunctionFRIH_HUMAN Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Publication Abstract from PubMedAuranofin (AF), a gold(I) compound that is currently used for the treatment of rheumatoid arthritis and is in clinical trials for its promising anticancer activity, was encapsulated within the human H-chain and the horse spleen ferritin nanocages using the alkaline disassembly/reassembly protocol. The aim of the work was to highlight possible differences in their drug loading capacity and efficacy. The drug-loaded ferritins were characterized via UV-vis absorption spectroscopy and inductively coupled plasma-atomic emission spectroscopy to assess AF encapsulation and to define the exact amount of gold atoms trapped in the Ft cavity. The crystal structures allowed us to define the nature of AF interaction with both ferritins and to identify the gold binding sites. Moreover, the biological characterization let us to obtain preliminary information on the cytotoxic effect of AF when bound to the human H-chain. Evaluation of Auranofin Loading within Ferritin Nanocages.,Lucignano R, Pratesi A, Imbimbo P, Monti DM, Picone D, Messori L, Ferraro G, Merlino A Int J Mol Sci. 2022 Nov 16;23(22):14162. doi: 10.3390/ijms232214162. PMID:36430642[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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