9s1f | pdb_00009s1f

Cryo-EM structure of activated retron Eco2 (Ec67)

spinqualitylabelsall models 9s1f, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9s1f is a 9 chain structure with sequence from Escherichia coli NCTC 86. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.9Å Ligands: MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RT67_ECOLX Reverse transcriptase (RT) component of antiviral defense system retron Ec67, minimally composed of a non-coding RNA (ncRNA) and this RT. Expression of these 2 elements confers protection against bacteriophage T5. At multiplicity of infection (MOI) of 0.02 cultures grow normally when infected with T5 without collapsing, at MOI 2 cultures enter growth stasis (PubMed:33157039). Responsible for synthesis of msDNA-Ec67 (a branched molecule with RNA linked by a 2',5'-phosphodiester bond to ssDNA). The retron transcript serves as primer (from a conserved internal G residue) and template for the reaction, and codes for the RT (PubMed:1378431, PubMed:1692831, PubMed:2466332). Can use other retrons as substrate (msDNA-Mx162 and msDNA-Ec86). Also able to synthesize DNA from a DNA template at least in vitro, although the enzyme is less active with a DNA template (PubMed:1692831).

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