2fqf

Crystal Structures of E. coli Laccase CueO under different copper binding situations

spinqualitylabelsall models 2fqf, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2fqf is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: C2O, CIT, CU Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CUEO_ECOLI Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed CueO protein is a hypothetical bacterial laccase and a good laccase candidate for large scale industrial application. Four CueO crystal structures were determined at different copper concentrations. Low copper occupancy in apo-CueO and slow copper reconstitution process in CueO with exogenous copper were demonstrated. These observations well explain the copper dependence of CueO oxidase activity. Structural comparison between CueO and other three fungal laccase proteins indicates that Glu106 in CueO constitutes the primary counter-work for reconstitution of the trinuclear copper site. Mutation of Glu106 to a Phe enhanced CueO oxidation activity and supported this hypothesis. In addition, an extra alpha-helix from Leu351 to Gly378 covers substrate biding pocket of CueO and might compromises the electron transfer from substrate to type I copper. Crystal structures of E. coli laccase CueO at different copper concentrations.,Li X, Wei Z, Zhang M, Peng X, Yu G, Teng M, Gong W Biochem Biophys Res Commun. 2007 Mar 2;354(1):21-6. Epub 2006 Dec 22. PMID:17217912 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Blue copper oxidase CueO Blue copper oxidase CueO 3D structures

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