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3r6s

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Crystal structure of GlxR transcription factor from Corynebacterium glutamicum with cAMP

Structural highlights

3r6s is a 6 chain structure with sequence from Corynebacterium glutamicum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.38Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CRPL_CORGL Global transcriptional regulator that complexes with cAMP and binds to specific DNA promoter sites, causing DNA-bending, to regulate transcription. cAMP improves binding to specific DNA sequences, probably by altering protein conformation. Involved in the regulation of gntP and gntK genes, which are involved in gluconate metabolism (PubMed:16385030). May form dimers which bind to the aceB promoter region in the presence of cAMP and repress the glyoxylate bypass genes (PubMed:15150232). It could be a positive regulator of rpf2 gene expression during growth on acetate as the sole carbon source, however because the cytosolic cAMP level is elevated in the presence of glucose and low upon growth on acetate, it is conceivable that it is unable to function as an activator under acetate conditions (PubMed:18355281).^[1] ^[2] ^[3]

References

↑ Kim HJ, Kim TH, Kim Y, Lee HS. Identification and characterization of glxR, a gene involved in regulation of glyoxylate bypass in Corynebacterium glutamicum. J Bacteriol. 2004 Jun;186(11):3453-60. doi: 10.1128/JB.186.11.3453-3460.2004. PMID:15150232 doi:http://dx.doi.org/10.1128/JB.186.11.3453-3460.2004
↑ Letek M, Valbuena N, Ramos A, Ordonez E, Gil JA, Mateos LM. Characterization and use of catabolite-repressed promoters from gluconate genes in Corynebacterium glutamicum. J Bacteriol. 2006 Jan;188(2):409-23. doi: 10.1128/JB.188.2.409-423.2006. PMID:16385030 doi:http://dx.doi.org/10.1128/JB.188.2.409-423.2006
↑ Jungwirth B, Emer D, Brune I, Hansmeier N, Puhler A, Eikmanns BJ, Tauch A. Triple transcriptional control of the resuscitation promoting factor 2 (rpf2) gene of Corynebacterium glutamicum by the regulators of acetate metabolism RamA and RamB and the cAMP-dependent regulator GlxR. FEMS Microbiol Lett. 2008 Apr;281(2):190-7. doi:, 10.1111/j.1574-6968.2008.01098.x. Epub 2008 Mar 18. PMID:18355281 doi:http://dx.doi.org/10.1111/j.1574-6968.2008.01098.x