Structural highlights
Function
TERT_ORYLA Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.[1] [2]
Publication Abstract from PubMed
Telomerase is a large ribonucleoprotein complex minimally composed of a catalytic telomerase reverse transcriptase (TERT) and an RNA component (TR) that provides the template for telomeric DNA synthesis. However, it remains unclear how TERT and TR assemble into a functional telomerase. Here we report the crystal structure of the conserved regions 4 and 5 (CR4/5) of TR in complex with the TR-binding domain (TRBD) of TERT from the teleost fish Oryzias latipes. The structure shows that CR4/5 adopts an L-shaped three-way-junction conformation with its two arms clamping onto TRBD. Both the sequence and conformation of CR4/5 are required for the interaction. Our structural and mutational analyses suggest that the observed CR4/5-TRBD recognition is common to most eukaryotes, and CR4/5 in vertebrate TR might have a similar role in telomerase regulation as that of stem-loop IV in Tetrahymena TR.
Structural basis for protein-RNA recognition in telomerase.,Huang J, Brown AF, Wu J, Xue J, Bley CJ, Rand DP, Wu L, Zhang R, Chen JJ, Lei M Nat Struct Mol Biol. 2014 Jun;21(6):507-12. doi: 10.1038/nsmb.2819. Epub 2014 May, 4. PMID:24793650[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Xie M, Mosig A, Qi X, Li Y, Stadler PF, Chen JJ. Structure and function of the smallest vertebrate telomerase RNA from teleost fish. J Biol Chem. 2008 Jan 25;283(4):2049-59. doi: 10.1074/jbc.M708032200. Epub 2007 , Nov 26. PMID:18039659 doi:http://dx.doi.org/10.1074/jbc.M708032200
- ↑ Bley CJ, Qi X, Rand DP, Borges CR, Nelson RW, Chen JJ. RNA-protein binding interface in the telomerase ribonucleoprotein. Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20333-8. doi: , 10.1073/pnas.1100270108. Epub 2011 Nov 28. PMID:22123986 doi:http://dx.doi.org/10.1073/pnas.1100270108
- ↑ Huang J, Brown AF, Wu J, Xue J, Bley CJ, Rand DP, Wu L, Zhang R, Chen JJ, Lei M. Structural basis for protein-RNA recognition in telomerase. Nat Struct Mol Biol. 2014 Jun;21(6):507-12. doi: 10.1038/nsmb.2819. Epub 2014 May, 4. PMID:24793650 doi:http://dx.doi.org/10.1038/nsmb.2819
