Structural highlights
Function
CLAMP_BPT4 Sliding clamp that encircles the genomic DNA and links the DNA polymerase to the template to control the processivity of DNA synthesis. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication (PubMed:10535942). Interaction with the sliding-clamp-loader opens the sliding clamp so that it can be loaded around the DNA template (PubMed:22194570). During transcription, encircles the DNA and tethers host RNA polymerase (RNAP) to it (PubMed:33602900).[HAMAP-Rule:MF_04161][1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Latham GJ, Dong F, Pietroni P, Dozono JM, Bacheller DJ, von Hippel PH. Opening of a monomer-monomer interface of the trimeric bacteriophage T4-coded GP45 sliding clamp is required for clamp loading onto DNA. Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12448-53. PMID:10535942 doi:10.1073/pnas.96.22.12448
- ↑ Kelch BA, Makino DL, O'Donnell M, Kuriyan J. How a DNA polymerase clamp loader opens a sliding clamp. Science. 2011 Dec 23;334(6063):1675-80. PMID:22194570 doi:10.1126/science.1211884
- ↑ Shi J, Wen A, Jin S, Gao B, Huang Y, Feng Y. Transcription activation by a sliding clamp. Nat Commun. 2021 Feb 18;12(1):1131. PMID:33602900 doi:10.1038/s41467-021-21392-0
