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|1gt0, resolution 2.60Å ()|
|Related:||1cqt, 1e3o, 1hf0, 1oct, 1pog, 1pou|
Crystal structure of a POU/HMG/DNA ternary complex
Members of the POU and SOX transcription factor families exemplify the partnerships established between various transcriptional regulators during early embryonic development. Although functional cooperativity between key regulator proteins is pivotal for milestone decisions in mammalian development, little is known about the underlying molecular mechanisms. In this study, we focus on two transcription factors, Oct4 and Sox2, as their combination on DNA is considered to direct the establishment of the first three lineages in the mammalian embryo. Using experimental high-resolution structure determination, followed by model building and experimental validation, we found that Oct4 and Sox2 were able to dimerize onto DNA in distinct conformational arrangements. We demonstrate that the DNA enhancer region of their target genes is responsible for the correct spatial alignment of glue-like interaction domains on their surface. Interestingly, these surfaces frequently have redundant functions and are instrumental in recruiting various interacting protein partners.
Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers., Remenyi A, Lins K, Nissen LJ, Reinbold R, Scholer HR, Wilmanns M, Genes Dev. 2003 Aug 15;17(16):2048-59. PMID:12923055
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1gt0 is a 4 chain structure with sequence from Homo sapiens and Mus musculus. The April 2009 RCSB PDB Molecule of the Month feature on Oct and Sox Transcription Factors by David Goodsell is 10.2210/rcsb_pdb/mom_2009_4. Full crystallographic information is available from OCA.