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1jrt
From Proteopedia
| 1jrt, resolution 1.70Å () | |||||||||
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| Ligands: | |||||||||
| Non-Standard Residues: | , | ||||||||
| Activity: | Trypsin, with EC number 3.4.21.4 | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
HEMIACETAL COMPLEX BETWEEN LEUPEPTIN AND TRYPSIN
Three-dimensional structures of trypsin with the reversible inhibitor leupeptin have been determined in two different crystal forms. The first structure was determined at 1.7 A resolution with R-factor = 17.7% in the trigonal crystal space group P3(1)21, with unit cell dimensions of a = b = 55.62 A, c = 110.51 A. The second structure was determined at a resolution of 1.8 A with R-factor = 17.5% in the orthorhombic space group P2(1)2(1)2(1), with unit cell dimensions of a = 63.69 A, b = 69.37 A, c = 63.01 A. The overall protein structure is very similar in both crystal forms, with RMS difference for main-chain atoms of 0.27 A. The leupeptin backbone forms four hydrogen bonds with trypsin and a fifth hydrogen bond interaction is mediated by a water molecule. The aldehyde carbonyl of leupeptin forms a covalent bond of 1.42 A length with side-chain oxygen of Ser-195 in the active site. The reaction of trypsin with leupeptin proceeds through the formation of stable tetrahedral complex in which the hemiacetal oxygen atom is pointing out of the oxyanion hole and forming a hydrogen bond with His-57.
Two crystal structures of the leupeptin-trypsin complex., Kurinov IV, Harrison RW, Protein Sci. 1996 Apr;5(4):752-8. PMID:8845765
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1jrt is a 2 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA.
See Also
Reference
- Kurinov IV, Harrison RW. Two crystal structures of the leupeptin-trypsin complex. Protein Sci. 1996 Apr;5(4):752-8. PMID:8845765
