1nj8

Structural highlights

Function

SYP_METJA Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). Can inadvertently accommodate and process non-cognate amino acids such as cysteine and alanine.^{[1] [2] [3] [4]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• Aminoacyl tRNA synthetase 3D structures

References

1. ↑ Stathopoulos C, Li T, Longman R, Vothknecht UC, Becker HD, Ibba M, Soll D. One polypeptide with two aminoacyl-tRNA synthetase activities. Science. 2000 Jan 21;287(5452):479-82. PMID:10642548
2. ↑ Lipman RS, Sowers KR, Hou YM. Synthesis of cysteinyl-tRNA(Cys) by a genome that lacks the normal cysteine-tRNA synthetase. Biochemistry. 2000 Jul 4;39(26):7792-8. PMID:10869184
3. ↑ Stathopoulos C, Jacquin-Becker C, Becker HD, Li T, Ambrogelly A, Longman R, Soll D. Methanococcus jannaschii prolyl-cysteinyl-tRNA synthetase possesses overlapping amino acid binding sites. Biochemistry. 2001 Jan 9;40(1):46-52. PMID:11141055
4. ↑ Lipman RS, Beuning PJ, Musier-Forsyth K, Hou YM. Amino acid activation of a dual-specificity tRNA synthetase is independent of tRNA. J Mol Biol. 2002 Feb 22;316(3):421-7. PMID:11866507 doi:http://dx.doi.org/10.1006/jmbi.2001.5373