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|1occ, resolution 2.80Å ()|
|Ligands:||, , ,|
STRUCTURE OF BOVINE HEART CYTOCHROME C OXIDASE AT THE FULLY OXIDIZED STATE
The crystal structure of bovine heart cytochrome c oxidase at 2.8 A resolution with an R value of 19.9 percent reveals 13 subunits, each different from the other, five phosphatidyl ethanolamines, three phosphatidyl glycerols and two cholates, two hemes A, and three copper, one magnesium, and one zinc. Of 3606 amino acid residues in the dimer, 3560 have been converged to a reasonable structure by refinement. A hydrogen-bonded system, including a propionate of a heme A (heme a), part of peptide backbone, and an imidazole ligand of CuA, could provide an electron transfer pathway between CuA and heme a. Two possible proton pathways for pumping, each spanning from the matrix to the cytosolic surfaces, were identified, including hydrogen bonds, internal cavities likely to contain water molecules, and structures that could form hydrogen bonds with small possible conformational change of amino acid side chains. Possible channels for chemical protons to produce H2O, for removing the produced water, and for O2, respectively, were identified.
The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A., Tsukihara T, Aoyama H, Yamashita E, Tomizaki T, Yamaguchi H, Shinzawa-Itoh K, Nakashima R, Yaono R, Yoshikawa S, Science. 1996 May 24;272(5265):1136-44. PMID:8638158
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
- Tsukihara T, Aoyama H, Yamashita E, Tomizaki T, Yamaguchi H, Shinzawa-Itoh K, Nakashima R, Yaono R, Yoshikawa S. The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 A. Science. 1996 May 24;272(5265):1136-44. PMID:8638158
- Tomizaki T, Yamashita E, Yamaguchi H, Aoyama H, Tsukihara T, Shinzawa-Itoh K, Nakashima R, Yaono R, Yoshikawa S. Structure analysis of bovine heart cytochrome c oxidase at 2.8 A resolution. Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):31-45. Epub 1999, Jan 1. PMID:10089392 doi:10.1107/S0907444998006362