Structural highlights
Function
THI4_ARATH Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Machado CR, de Oliveira RL, Boiteux S, Praekelt UM, Meacock PA, Menck CF. Thi1, a thiamine biosynthetic gene in Arabidopsis thaliana, complements bacterial defects in DNA repair. Plant Mol Biol. 1996 Jun;31(3):585-93. PMID:8790291
- ↑ Papini-Terzi FS, Galhardo RS, Farias LP, Menck CF, Van Sluys MA. Point mutation is responsible for Arabidopsis tz-201 mutant phenotype affecting thiamin biosynthesis. Plant Cell Physiol. 2003 Aug;44(8):856-60. PMID:12941878
