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1t2k
From Proteopedia
Structure Of The DNA Binding Domains Of IRF3, ATF-2 and Jun Bound To DNA
Transcriptional activation of the interferon-beta (IFN-beta) gene requires assembly of an enhanceosome containing the transcription factors ATF-2/c-Jun, IRF-3/IRF-7, NF-kappaB and HMGI(Y). These factors cooperatively bind a composite DNA site and activate expression of the IFN-beta gene. The 3.0 A crystal structure of the DNA-binding domains of ATF-2/c-Jun and two IRF-3 molecules in a complex with 31 base pairs (bp) of the PRDIV-PRDIII region of the IFN-beta enhancer shows that association of the four proteins with DNA creates a continuous surface for the recognition of 24 bp. The structure, together with in vitro binding studies and protein mutagenesis, shows that protein-protein interactions are not critical for cooperative binding. Instead, cooperativity arises mainly through nucleotide sequence-dependent structural changes in the DNA that allow formation of complementary DNA conformations. Because the binding sites overlap on the enhancer, the unit of recognition is the entire nucleotide sequence, not the individual subsites.
Crystal structure of ATF-2/c-Jun and IRF-3 bound to the interferon-beta enhancer., Panne D, Maniatis T, Harrison SC, EMBO J. 2004 Nov 10;23(22):4384-93. Epub 2004 Oct 28. PMID:15510218
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1T2K is a 6 chains structure with sequences from Homo sapiens. The February 2010 RCSB PDB Molecule of the Month feature on Enhanceosome by David Goodsell is 10.2210/rcsb_pdb/mom_2010_2. Full crystallographic information is available from OCA.
Reference
- Panne D, Maniatis T, Harrison SC. Crystal structure of ATF-2/c-Jun and IRF-3 bound to the interferon-beta enhancer. EMBO J. 2004 Nov 10;23(22):4384-93. Epub 2004 Oct 28. PMID:15510218
Page seeded by OCA on Wed Jan 20 22:14:33 2010
