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|1x8w, resolution 3.80Å ()|
Structure of the Tetrahymena Ribozyme: Base Triple Sandwich and Metal Ion at the Active Site
The Tetrahymena intron is an RNA catalyst, or ribozyme. As part of its self-splicing reaction, this ribozyme catalyzes phosphoryl transfer between guanosine and a substrate RNA strand. Here we report the refined crystal structure of an active Tetrahymena ribozyme in the absence of its RNA substrate at 3.8 A resolution. The 3'-terminal guanosine (omegaG), which serves as the attacking group for RNA cleavage, forms a coplanar base triple with the G264-C311 base pair, and this base triple is sandwiched by three other base triples. In addition, a metal ion is present in the active site, contacting or positioned close to the ribose of the omegaG and five phosphates. All of these phosphates have been shown to be important for catalysis. Therefore, we provide a picture of how the ribozyme active site positions both a catalytic metal ion and the nucleophilic guanosine for catalysis prior to binding its RNA substrate.
Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site., Guo F, Gooding AR, Cech TR, Mol Cell. 2004 Nov 5;16(3):351-62. PMID:15525509
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1x8w is a 4 chain structure. Full crystallographic information is available from OCA.
- Guo F, Gooding AR, Cech TR. Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site. Mol Cell. 2004 Nov 5;16(3):351-62. PMID:15525509 doi:S1097276504005921
- Hougland JL, Kravchuk AV, Herschlag D, Piccirilli JA. Functional identification of catalytic metal ion binding sites within RNA. PLoS Biol. 2005 Sep;3(9):e277. Epub 2005 Aug 16. PMID:16092891 doi:10.1371/journal.pbio.0030277
- Chadee AB, Bhaskaran H, Russell R. Protein roles in group I intron RNA folding: the tyrosyl-tRNA synthetase CYT-18 stabilizes the native state relative to a long-lived misfolded structure without compromising folding kinetics. J Mol Biol. 2010 Jan 22;395(3):656-70. Epub 2009 Nov 11. PMID:19913030 doi:10.1016/j.jmb.2009.11.009