1z24
From Proteopedia
The molecular structure of insecticyanin from the tobacco hornworm Manduca sexta L. at 2.6 A resolution.
Structural highlights
FunctionICYA_MANSE This protein binds a chromophore: biliverdin IX, isomer gamma. Mixed with lipoprotein-bound carotenes, this blue protein provides hornworms with their green cryptic coloration which serves a camouflage. Evolutionary ConservationCheck, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedInsecticyanin, a blue biliprotein isolated from the tobacco hornworm Manduca sexta L., is involved in insect camouflage. Its three-dimensional structure has now been solved to 2.6 A resolution using the techniques of multiple isomorphous replacement, non-crystallographic symmetry averaging about a local 2-fold rotation axis and solvent flattening. All 189 amino acids have been fitted to the electron density map. The map clearly shows that insecticyanin is a tetramer with one of its molecular 2-fold axes coincident to a crystallographic dyad. The individual subunits have overall dimensions of 44 A X 37 A X 40 A and consist primarily of an eight-stranded anti-parallel beta-barrel flanked on one side by a 4.5-turn alpha-helix. Interestingly the overall three-dimensional fold of the insecticyanin subunit shows remarkable similarity to the structural motifs of bovine beta-lactoglobulin and the human serum retinol-binding protein. The electron density attributable to the chromophore is unambiguous and shows that it is indeed the gamma-isomer of biliverdin. The biliverdin lies towards the open end of the beta-barrel with its two propionate side chains pointing towards the solvent and it adopts a rather folded conformation, much like a heme. The molecular structure of insecticyanin from the tobacco hornworm Manduca sexta L. at 2.6 A resolution.,Holden HM, Rypniewski WR, Law JH, Rayment I EMBO J. 1987 Jun;6(6):1565-70. PMID:3608987[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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