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2he2
From Proteopedia
| 2he2, resolution 1.50Å () | |||||||||
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| Gene: | DLG2 (Homo sapiens) | ||||||||
| Domains: | PDZ_signaling | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal structure of the 3rd PDZ domain of human discs large homologue 2, DLG2
PDZ domains are protein-protein interaction modules that generally bind to the C termini of their target proteins. The C-terminal four amino acids of a prospective binding partner of a PDZ domain are typically the determinants of binding specificity. In an effort to determine the structures of a number of PDZ domains we have included appropriate four residue extensions on the C termini of PDZ domain truncation mutants, designed for self-binding. Multiple truncations of each PDZ domain were generated. The four residue extensions, which represent known specificity sequences of the target PDZ domains and cover both class I and II motifs, form intermolecular contacts in the expected manner for the interactions of PDZ domains with protein C termini for both classes. We present the structures of eight unique PDZ domains crystallized using this approach and focus on four which provide information on selectivity (PICK1 and the third PDZ domain of DLG2), binding site flexibility (the third PDZ domain of MPDZ), and peptide-domain interactions (MPDZ 12th PDZ domain). Analysis of our results shows a clear improvement in the chances of obtaining PDZ domain crystals by using this approach compared to similar truncations of the PDZ domains without the C-terminal four residue extensions.
Structure of PICK1 and other PDZ domains obtained with the help of self-binding C-terminal extensions., Elkins JM, Papagrigoriou E, Berridge G, Yang X, Phillips C, Gileadi C, Savitsky P, Doyle DA, Protein Sci. 2007 Apr;16(4):683-94. PMID:17384233
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2HE2 is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Elkins JM, Papagrigoriou E, Berridge G, Yang X, Phillips C, Gileadi C, Savitsky P, Doyle DA. Structure of PICK1 and other PDZ domains obtained with the help of self-binding C-terminal extensions. Protein Sci. 2007 Apr;16(4):683-94. PMID:17384233 doi:16/4/683
Page seeded by OCA on Mon Feb 16 15:57:33 2009
Categories: Homo sapiens | Arrowsmith, C. | Berridge, G. | Debreczeni, J. | Delft, F von. | Doyle, D A. | Edwards, A. | Elkins, J M. | Gorrec, F. | Papagrigoriou, E. | Phillips, C. | SGC, Structural Genomics Consortium. | Savitsky, P. | Smee, C E.A. | Sundstrom, M. | Turnbull, A P. | Weigelt, J. | Dlg2 | Pdz | Pdz domain | Sgc | Signal transduction | Signaling protein | Structural genomic | Structural genomics consortium
