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2he7
From Proteopedia
| 2he7, resolution 2.00Å () | |||||||||
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| Gene: | EPB41L3 (Homo sapiens) | ||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
FERM domain of EPB41L3 (DAL-1)
Perturbed cell-adhesion mechanisms are crucial for tumor invasion and metastasis. A cell-adhesion protein, Tumor Suppressor in Lung Cancer 1 (TSLC1), is inactivated in a majority of metastatic cancers. DAL-1 (differentially expressed in adenocarcinoma of the lung protein), another tumor suppressor, binds through its FERM domain to the TSLC1 C-terminal, 4.1 glycophorin C-like, cytoplasmic domain. However, the molecular basis for this interaction is unknown. Here, we describe the crystal structure of a complex between the DAL-1 FERM domain and a portion of the TSLC1 cytoplasmic domain. DAL-1 binds to TSLC1 through conserved residues in a well-defined hydrophobic pocket in the DAL-1 FERM domain's structural C-lobe. From the crystal structure, it is apparent that Tyr406 and Thr408 in the TSLC1 cytoplasmic domain form the most important interactions with DAL-1 and this was also confirmed by surface plasmon resonance studies. Our results refute earlier exon deletion experiments that indicated that glycophorin-C interacts with the alpha-lobe of 4.1 FERM domains.
Structural basis of tumor suppressor in lung cancer 1 (TSLC1) binding to differentially expressed in adenocarcinoma of the lung (DAL-1/4.1B)., Busam RD, Thorsell AG, Flores A, Hammarstrom M, Persson C, Obrink B, Hallberg BM, J Biol Chem. 2010 Dec 3. PMID:21131357
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2he7 is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Busam RD, Thorsell AG, Flores A, Hammarstrom M, Persson C, Obrink B, Hallberg BM. Structural basis of tumor suppressor in lung cancer 1 (TSLC1) binding to differentially expressed in adenocarcinoma of the lung (DAL-1/4.1B). J Biol Chem. 2010 Dec 3. PMID:21131357 doi:10.1074/jbc.M110.174011
Categories: Homo sapiens | Arrowsmith, C. | Berglund, H. | Busam, R D. | Collins, R. | Edwards, A. | Ehn, M. | Flodin, S. | Flores, A. | Graslund, S. | Hallberg, B M. | Hammarstrom, M. | Hogbom, M. | Johansson, I. | Karlberg, T. | Kotenyova, T. | Nilsson-ehle, P. | Nilvebrandt, J. | Norberg, P. | Nordlund, P. | Nyman, T. | Ogg, D. | Persson, C. | SGC, Structural Genomics Consortium. | Sagemark, J. | Schiavone, L H. | Stenmark, P. | Sundstrom, M. | Thorsell, A G. | Uppenberg, J. | Weigelt, J. | Berg, S Van den. | Cell adhesion | Dal-1 | Epb41l3a | Ferm domain | Sgc | Structural genomic | Structural genomics consortium
