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2ihu
From Proteopedia
| 2ihu, resolution 2.05Å () | |||||||||
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| Ligands: | , , , , , | ||||||||
| Gene: | ceas2 (Streptomyces clavuligerus) | ||||||||
| Activity: | N(2)-(2-carboxyethyl)arginine synthase, with EC number 2.5.1.66 | ||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Carboxyethylarginine synthase from Streptomyces clavuligerus: putative reaction intermediate complex
N(2)-(2-Carboxyethyl)arginine synthase (CEAS), an unusual thiamin diphosphate (ThDP)-dependent enzyme, catalyses the committed step in the biosynthesis of the b-lactamase inhibitor clavulanic acid in Streptomyces clavuligerus. Crystal structures of tetrameric CEAS-ThDP in complex with the substrate analogues 5-guanidinovaleric acid (GVA) and tartrate, and a structure reflecting a possible enol(ate)-ThDP reaction intermediate are described. The structures suggest overlapping binding sites for the substrates D-glyceraldehyde-3-phosphate (D-G3P) and L-arginine, and are consistent with the proposed CEAS mechanism in which D-G3P binds at the active site and reacts to form an alpha,beta-unsaturated intermediate,which subsequently undergoes (1,4)-Michael addition with the alpha-amino group of L-arginine. Additional solution studies are presented which probe the amino acid substrate tolerance of CEAS, providing further insight into the L-arginine binding site. These findings may facilitate the engineering of CEAS towards the synthesis of alternative beta-amino acid products.
Structural and mechanistic studies on N(2)-(2-carboxyethyl)arginine synthase., Caines ME, Sorensen JL, Schofield CJ, Biochem Biophys Res Commun. 2009 Aug 7;385(4):512-7. Epub 2009 May 27. PMID:19477162
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2ihu is a 4 chain structure with sequence from Streptomyces clavuligerus. Full crystallographic information is available from OCA.
Reference
- Caines ME, Sorensen JL, Schofield CJ. Structural and mechanistic studies on N(2)-(2-carboxyethyl)arginine synthase. Biochem Biophys Res Commun. 2009 Aug 7;385(4):512-7. Epub 2009 May 27. PMID:19477162 doi:10.1016/j.bbrc.2009.05.095
