2jln
From Proteopedia
Structure of Mhp1, a nucleobase-cation-symport-1 family transporter
Structural highlights
FunctionHYUP_MICLQ Nucleobase-proton symporter that mediates the sodium-dependent binding and uptake of 5-aryl-substituted hydantoin compounds (PubMed:16621827, PubMed:24952894). 5-indolyl methyl hydantoin and 5-benzyl hydantoin are the preferred substrates, with selectivity for a hydrophobic substituent in position 5 of hydantoin and for the L isomer over the D isomer (PubMed:16621827, PubMed:24952894).[1] [2] [3] Evolutionary ConservationCheck, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe 'Nucleobase-Cation-Symport-1', NCS1, transporters are essential components of salvage pathways for nucleobases and related metabolites. Here, we report the 2.85 A resolution structure of the NCS1 benzyl-hydantoin transporter, Mhp1, from Microbacterium liquefaciens. Mhp1 contains 12 transmembrane helices, 10 of which are arranged in two inverted repeats of 5 helices. The structures of the outward-facing open and substrate-bound occluded conformations were solved, showing how the outward-facing cavity closes upon binding of substrate. Comparisons with the leucine (LeuTAa) and the galactose (vSGLT) transporters reveal that the outward- and inward-facing cavities are symmetrically arranged on opposite sides of the membrane. The reciprocal opening and closing of these cavities is synchronised by the inverted repeat helices 3 and 8, providing the structural basis of the 'alternating access' model for membrane transport. Structure and Molecular Mechanism of a Nucleobase-Cation-Symport-1 Family Transporter.,Weyand S, Shimamura T, Yajima S, Suzuki S, Mirza O, Krusong K, Carpenter EP, Rutherford NG, Hadden JM, O'Reilly J, Ma P, Saidijam M, Patching SG, Hope RJ, Norbertczak HT, Roach PC, Iwata S, Henderson PJ, Cameron AD Science. 2008 Oct 16. PMID:18927357[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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Categories: Large Structures | Microbacterium liquefaciens | Cameron AD | Carpenter EP | Hadden JM | Henderson PJF | Hope RJ | Iwata S | Krusong K | Ma P | Mirza O | Norbertczak HT | O'Reilly J | Patching SG | Roach PCJ | Rutherford NG | Saidijam M | Shimamura T | Suzuki S | Weyand S | Yajima S