2ote

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Crystal structure of a monomeric cyan fluorescent protein in the photobleached state

Structural highlights

2ote is a 2 chain structure with sequence from Clavularia sp.. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.47Å
Ligands:ACT, PIA
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GFPL_CLASP

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Fluorescent protein (FP) variants that can be reversibly converted between fluorescent and nonfluorescent states have proven to be a catalyst for innovation in the field of fluorescence microscopy. However, the structural basis of the process remains poorly understood. High-resolution structures of a FP derived from Clavularia in both the fluorescent and the light-induced nonfluorescent states reveal that the rapid and complete loss of fluorescence observed upon illumination with 450-nm light results from cis-trans isomerization of the chromophore. The photoinduced change in configuration from the well ordered cis isomer to the highly nonplanar and disordered trans isomer is accompanied by a dramatic rearrangement of internal side chains. Taken together, the structures provide an explanation for the loss of fluorescence upon illumination, the slow light-independent recovery, and the rapid light-induced recovery of fluorescence. The fundamental mechanism appears to be common to all of the photoactivatable and reversibly photoswitchable FPs reported to date.

Structural basis for reversible photobleaching of a green fluorescent protein homologue.,Henderson JN, Ai HW, Campbell RE, Remington SJ Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6672-7. Epub 2007 Apr 9. PMID:17420458[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
34 reviews cite this structure
Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
33 more
75 other articles
No citations found

See Also

References

  1. ↑ Henderson JN, Ai HW, Campbell RE, Remington SJ. Structural basis for reversible photobleaching of a green fluorescent protein homologue. Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6672-7. Epub 2007 Apr 9. PMID:17420458

Contents


PDB ID 2ote

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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