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2qe3
From Proteopedia
| 2qe3, resolution 2.50Å () | |||||||||
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| Gene: | TNFSF15 (Homo sapiens) | ||||||||
| Domains: | TNF | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal structure of human tl1a extracellular domain
TNF-like 1A (TL1A) is a newly described member of the TNF superfamily that is directly implicated in the pathogenesis of autoimmune diseases, including inflammatory bowel disease, atherosclerosis, and rheumatoid arthritis. We report the crystal structure of the human TL1A extracellular domain at a resolution of 2.5 A, which reveals a jelly-roll fold typical of the TNF superfamily. This structural information, in combination with complementary mutagenesis and biochemical characterization, provides insights into the binding interface and the specificity of the interactions between TL1A and the DcR3 and DR3 receptors. These studies suggest that the mode of interaction between TL1A and DcR3 differs from other characterized TNF ligand/receptor complexes. In addition, we have generated functional TL1A mutants with altered disulfide bonding capability that exhibit enhanced solution properties, which will facilitate the production of materials for future cell-based and whole animal studies. In summary, these studies provide insights into the structure and function of TL1A and provide the basis for the rational manipulation of its interactions with cognate receptors.
Biochemical and structural characterization of the human TL1A ectodomain., Zhan C, Yan Q, Patskovsky Y, Li Z, Toro R, Meyer A, Cheng H, Brenowitz M, Nathenson SG, Almo SC, Biochemistry. 2009 Aug 18;48(32):7636-45. PMID:19522538
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
2QE3 is a 1 chain structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Zhan C, Yan Q, Patskovsky Y, Li Z, Toro R, Meyer A, Cheng H, Brenowitz M, Nathenson SG, Almo SC. Biochemical and structural characterization of the human TL1A ectodomain. Biochemistry. 2009 Aug 18;48(32):7636-45. PMID:19522538 doi:10.1021/bi900031w
Page seeded by OCA on Mon Jan 18 09:47:51 2010
Categories: Homo sapiens | Almo, S C. | Bonanno, J. | Nathenson, S G. | Patskovsky, Y. | Shi, W. | Toro, R. | Yan, Q. | Zhan, C. | Cytokine | Tl1a | Tnfsf
