2w7z

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Structure of the pentapeptide repeat protein EfsQnr, a DNA gyrase inhibitor. Free amines modified by cyclic pentylation with glutaraldehyde.

Structural highlights

2w7z is a 2 chain structure with sequence from Enterococcus faecalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Ligands:CL, LP6
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q837D8_ENTFA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The pentapeptide-repeat protein EfsQnr from Enterococcus faecalis protects DNA gyrase from inhibition by fluoroquinolones. EfsQnr was cloned and purified to homogeneity, but failed to produce diffraction-quality crystals in initial crystallization screens. Treatment of EfsQnr with glutaraldehyde and the strong reducing agent borane-dimethylamine resulted in a derivatized protein which produced crystals that diffracted to 1.6 A resolution; their structure was subsequently determined by single-wavelength anomalous dispersion. Analysis of the derivatized protein using Fourier transform ion cyclotron resonance mass spectrometry indicated a mass increase of 68 Da per free amino group. Electron-density maps about a limited number of structurally ordered lysines indicated that the modification was a cyclic pentylation of free amines, producing piperidine groups.

Crystallization of a pentapeptide-repeat protein by reductive cyclic pentylation of free amines with glutaraldehyde.,Vetting MW, Hegde SS, Blanchard JS Acta Crystallogr D Biol Crystallogr. 2009 May;65(Pt 5):462-9. Epub 2009, Apr 18. PMID:19390151[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
1 reviews cite this structure
Current Understanding of the Structure and Function of Pentapeptide Repeat Proteins.
Zhang et al. (2021)
0 more
2 other articles
No citations found

References

  1. ↑ Vetting MW, Hegde SS, Blanchard JS. Crystallization of a pentapeptide-repeat protein by reductive cyclic pentylation of free amines with glutaraldehyde. Acta Crystallogr D Biol Crystallogr. 2009 May;65(Pt 5):462-9. Epub 2009, Apr 18. PMID:19390151 doi:10.1107/S0907444909008324

Contents


PDB ID 2w7z

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