2w88

From Proteopedia

Plastocyanin variant with N-terminal Methionine - open structure

Structural highlights

2w88 is a 3 chain structure with sequence from Phormidium laminosum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.89Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PLAS_PHOLA Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The beta-sandwich cupredoxin Plastocyanin (Pc) was found to self-assemble in the presence of Zn(2+), a known mediator of protein-protein interfaces. Diffraction-quality crystals of Pc grew from solutions containing zinc acetate as the sole precipitant. Di- and trinuclear zinc sites contribute to the crystal contacts in this structure. A different crystal form, also involving numerous zinc bridging ions, was obtained in the presence of poly(ethylene glycol) 8 000. Comparison of the two crystal forms reveals the effect of macromolecular crowding on self-assembly. Solution-state structural characterisation of the Zn(2+)-mediated Pc oligomers was performed by using a combination of chemical shift perturbation mapping and small-angle X-ray scattering. The data indicate the formation of dimers in solution. The implications for metal-mediated assembly and crystallisation are discussed.

Metal-Mediated Self-Assembly of a beta-Sandwich Protein.,Crowley PB, Matias PM, Khan AR, Roessle M, Svergun DI Chemistry. 2009 Oct 15. PMID:19834935^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Crowley PB, Matias PM, Khan AR, Roessle M, Svergun DI. Metal-Mediated Self-Assembly of a beta-Sandwich Protein. Chemistry. 2009 Oct 15. PMID:19834935 doi:10.1002/chem.200901410