3bw3

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Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes

Structural highlights

3bw3 is a 1 chain structure with sequence from Streptomyces ansochromogenes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:FMN, MPD, NIE
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9FDD4_9ACTN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Nitroalkane oxidase (NAO) catalyzes neutral nitroalkanes to their corresponding aldehydes or ketones, hydrogen peroxide and nitrite. The crystal structure of NAO from Streptomyces ansochromogenes was determined; it consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a novel folding pattern. Site-directed mutagenesis of His179, which is spatially adjacent to FMN, resulted in the loss of enzyme activity, demonstrating that this amino acid residue is important for catalysis. The crystal structure of mutant H179D-nitroethane was also analyzed. Interestingly, Sa-NAO shows the typical function as nitroalkane oxidase but its structure is similar to that of 2-nitropropane dioxygenase. Overall, these results suggest that Sa-NAO is a novel nitroalkane oxidase with TIM barrel structure.

Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes.,Li Y, Gao Z, Hou H, Li L, Zhang J, Yang H, Dong Y, Tan H Biochem Biophys Res Commun. 2011 Feb 18;405(3):344-8. Epub 2010 Dec 11. PMID:21147069[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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References

  1. ↑ Li Y, Gao Z, Hou H, Li L, Zhang J, Yang H, Dong Y, Tan H. Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes. Biochem Biophys Res Commun. 2011 Feb 18;405(3):344-8. Epub 2010 Dec 11. PMID:21147069 doi:10.1016/j.bbrc.2010.12.050

Contents


PDB ID 3bw3

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