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3crx
From Proteopedia
| 3crx, resolution 2.50Å () | |||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
CRE RECOMBINASE/DNA COMPLEX INTERMEDIATE I
We have determined the X-ray crystal structures of two DNA Holliday junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched structure that occurs as an intermediate in genetic recombination pathways, including site-specific recombination by the lambda-integrase family. Cre recombinase is an integrase family member that recombines 34 bp loxP sites in the absence of accessory proteins or auxiliary DNA sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ reveal a nearly planar, twofold-symmetric DNA intermediate that shares features with both the stacked-X and the square conformations of the HJ that exist in the unbound state. The structures support a protein-mediated crossover isomerization of the junction that acts as the switch responsible for activation and deactivation of recombinase active sites. In this model, a subtle isomerization of the Cre recombinase-HJ quaternary structure dictates which strands are cleaved during resolution of the junction via a mechanism that involves neither branch migration nor helical restacking.
Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination., Gopaul DN, Guo F, Van Duyne GD, EMBO J. 1998 Jul 15;17(14):4175-87. PMID:9670032
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3crx is a 6 chain structure with sequence from Enterobacteria phage p1. Full crystallographic information is available from OCA.
See Also
Reference
- Gopaul DN, Guo F, Van Duyne GD. Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination. EMBO J. 1998 Jul 15;17(14):4175-87. PMID:9670032 doi:10.1093/emboj/17.14.4175
