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3dv2
From Proteopedia
| 3dv2, resolution 2.30Å () | |||||||||
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| Ligands: | |||||||||
| Gene: | nadD (Bacillus anthracis) | ||||||||
| Activity: | Nicotinate-nucleotide adenylyltransferase, with EC number 2.7.7.18 | ||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Crystal Structure of nicotinic acid mononucleotide adenylyltransferase from Bacillus anthracis
Nicotinic acid mononucleotide adenylyltransferase (NaMNAT; EC 2.7.7.18) is the penultimate enzyme in the biosynthesis of NAD(+) and catalyzes the adenylation of nicotinic acid mononucleotide (NaMN) by ATP to form nicotinic acid adenine dinucleotide (NaAD). This enzyme is regarded as a suitable candidate for antibacterial drug development; as such, Bacillus anthracis NaMNAT (BA NaMNAT) was heterologously expressed in Escherichia coli for the purpose of inhibitor discovery and crystallography. The crystal structure of BA NaMNAT was determined by molecular replacement, revealing two dimers per asymmetric unit, and was refined to an R factor and R(free) of 0.228 and 0.263, respectively, at 2.3 A resolution. The structure is very similar to that of B. subtilis NaMNAT (BS NaMNAT), which is also a dimer, and another independently solved structure of BA NaMNAT recently released from the PDB along with two ligated forms. Comparison of these and other less related bacterial NaMNAT structures support the presence of considerable conformational heterogeneity and flexibility in three loops surrounding the substrate-binding area.
Structure of nicotinic acid mononucleotide adenylyltransferase from Bacillus anthracis., Lu S, Smith CD, Yang Z, Pruett PS, Nagy L, McCombs D, Delucas LJ, Brouillette WJ, Brouillette CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Oct 1;64(Pt, 10):893-8. Epub 2008 Sep 30. PMID:18931430
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3dv2 is a 4 chain structure with sequence from Bacillus anthracis. Full crystallographic information is available from OCA.
Reference
- Lu S, Smith CD, Yang Z, Pruett PS, Nagy L, McCombs D, Delucas LJ, Brouillette WJ, Brouillette CG. Structure of nicotinic acid mononucleotide adenylyltransferase from Bacillus anthracis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Oct 1;64(Pt, 10):893-8. Epub 2008 Sep 30. PMID:18931430 doi:10.1107/S1744309108029102
Categories: Bacillus anthracis | Nicotinate-nucleotide adenylyltransferase | Brouillette, C G. | Brouillette, W J. | DeLucas, L J. | Lu, S. | McCombs, D P. | Nagy, L. | Pruett, P S. | Smith, C D. | Yang, Z. | Alpha and beta protein | Nad | Nucleotidyltransferase | Pyridine nucleotide biosynthesis | Rossman fold | Transferase
