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3g6x

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3g6x, resolution 2.08Å ()
Ligands: ,
Non-Standard Residues: ,
Gene: POLI, RAD30B (Homo sapiens)
Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7
Related: 2fll, 3g6v, 3g6y
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Ternary complex of DNA Polymerase iota:DNA:dGTP with an abasic site at the templating position

Publication Abstract from PubMed

Abasic sites are among the most abundant DNA lesions formed in human cells, and they present a strong block to replication. DNA polymerase iota (Poliota) is one of the few DNA Pols that does not follow the A-rule opposite an abasic site. We present here three structures of human Poliota in complex with DNAs containing an abasic lesion and dGTP, dTTP, or dATP as the incoming nucleotide. The structures reveal a mechanism of translesion synthesis across an abasic lesion that differs from that in other Pols. Both the abasic lesion and the incoming dNTPs are intrahelical and are closely apposed across a constricted active site cleft. The dNTPs partake in distinct networks of hydrogen bonds in the "void" opposite the lesion. These different patterns of hydrogen bonds, as well as stacking interactions, may underlie Poliota's small preference for insertion of dGTP over other nucleotides opposite this common lesion.

DNA synthesis across an abasic lesion by human DNA polymerase iota., Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK, Structure. 2009 Apr 15;17(4):530-7. PMID:19368886

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3g6x is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

See Also

Reference

  • Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK. DNA synthesis across an abasic lesion by human DNA polymerase iota. Structure. 2009 Apr 15;17(4):530-7. PMID:19368886 doi:10.1016/j.str.2009.02.015

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