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3g6y
From Proteopedia
| 3g6y, resolution 2.10Å () | |||||||||
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| Ligands: | , | ||||||||
| Non-Standard Residues: | , | ||||||||
| Gene: | POLI, RAD30B (Homo sapiens) | ||||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||||
| Related: | 2fll, 3g6v, 3g6x | ||||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
Ternary complex of DNA Polymerase iota:DNA:dTTP with an abasic site at the templating position
Abasic sites are among the most abundant DNA lesions formed in human cells, and they present a strong block to replication. DNA polymerase iota (Poliota) is one of the few DNA Pols that does not follow the A-rule opposite an abasic site. We present here three structures of human Poliota in complex with DNAs containing an abasic lesion and dGTP, dTTP, or dATP as the incoming nucleotide. The structures reveal a mechanism of translesion synthesis across an abasic lesion that differs from that in other Pols. Both the abasic lesion and the incoming dNTPs are intrahelical and are closely apposed across a constricted active site cleft. The dNTPs partake in distinct networks of hydrogen bonds in the "void" opposite the lesion. These different patterns of hydrogen bonds, as well as stacking interactions, may underlie Poliota's small preference for insertion of dGTP over other nucleotides opposite this common lesion.
DNA synthesis across an abasic lesion by human DNA polymerase iota., Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK, Structure. 2009 Apr 15;17(4):530-7. PMID:19368886
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3g6y is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.
See Also
Reference
- Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK. DNA synthesis across an abasic lesion by human DNA polymerase iota. Structure. 2009 Apr 15;17(4):530-7. PMID:19368886 doi:10.1016/j.str.2009.02.015
Categories: DNA-directed DNA polymerase | Homo sapiens | Aggarwal, A K. | Nair, D T. | Abasic site | Dna damage | Dna polymerase | Dna repair | Dna replication | Dna synthesis | Dna-binding | Dna-directed dna polymerase | Lesion bypass | Magnesium | Metal-binding | Mutator protein | Nucleotidyltransferase | Nucleus | Replication-dna complex | Schiff base | Ternary complex | Transferase | Y-family
