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3gj8

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3gj8, resolution 1.82Å ()
Ligands: , ,
Gene: RAN, ARA24, OK/SW-cl.81 (Homo sapiens), Nup153 (Rattus norvegicus)
Related: 3ch5, 2gqe, 1byu, 3gj0, 3gj3, 3gj4, 3gj5, 3gj6, 3gj7
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Crystal structure of human RanGDP-Nup153ZnF34 complex

Publication Abstract from PubMed

The nuclear pore complex (NPC) resides in circular openings within the nuclear envelope and serves as the sole conduit to facilitate nucleocytoplasmic transport in eukaryotes. The asymmetric distribution of the small G protein Ran across the nuclear envelope regulates directionality of protein transport. Ran interacts with the NPC of metazoa via two asymmetrically localized components, Nup153 at the nuclear face and Nup358 at the cytoplasmic face. Both nucleoporins contain a stretch of distinct, Ran-binding zinc finger domains. Here, we present six crystal structures of Nup153-zinc fingers in complex with Ran and a 1.48 A crystal structure of RanGDP. Crystal engineering allowed us to obtain well diffracting crystals so that all ZnF-Ran complex structures are refined to high resolution. Each of the four zinc finger modules of Nup153 binds one Ran molecule in apparently non-allosteric fashion. The affinity is measurably higher for RanGDP than for RanGTP and varies modestly between the individual zinc fingers. By microcalorimetric and mutational analysis, we determined that one specific hydrogen bond accounts for most of the differences in the binding affinity of individual zinc fingers. Genomic analysis reveals that only in animals do NPCs contain Ran-binding zinc fingers. We speculate that these organisms evolved a mechanism to maintain a high local concentration of Ran at the vicinity of the NPC, using this zinc finger domain as a sink.

Crystallographic and biochemical analysis of the Ran-binding zinc finger domain., Partridge JR, Schwartz TU, J Mol Biol. 2009 Aug 14;391(2):375-89. Epub 2009 Jun 6. PMID:19505478

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3gj8 is a 4 chain structure with sequence from Homo sapiens and Rattus norvegicus. Full crystallographic information is available from OCA.

See Also

Reference

  • Partridge JR, Schwartz TU. Crystallographic and biochemical analysis of the Ran-binding zinc finger domain. J Mol Biol. 2009 Aug 14;391(2):375-89. Epub 2009 Jun 6. PMID:19505478 doi:10.1016/j.jmb.2009.06.011

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