3h0d

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Crystal structure of CtsR in complex with a 26bp DNA duplex

Structural highlights

3h0d is a 4 chain structure with sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:MSE, PO4
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CTSR_GEOSE Controls the expression of the cellular protein quality control genes clpC, clpE and clpP, as well as mcsA and mcsB, by acting as a repressor of these class III stress genes. After heat shock, CtsR is degraded by the ClpCP and ClpEP proteolytic systems, ensuring the derepression of clpE, clpP and the clpC operon. CtsR negatively autoregulates its own synthesis (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

All living organisms face a variety of environmental stresses that cause the misfolding and aggregation of proteins. To eliminate damaged proteins, cells developed highly efficient stress response and protein quality control systems. We performed a biochemical and structural analysis of the bacterial CtsR/McsB stress response. The crystal structure of the CtsR repressor, in complex with DNA, pinpointed key residues important for high-affinity binding to the promoter regions of heat-shock genes. Moreover, biochemical characterization of McsB revealed that McsB specifically phosphorylates arginine residues in the DNA binding domain of CtsR, thereby impairing its function as a repressor of stress response genes. Identification of the CtsR/McsB arginine phospho-switch expands the repertoire of possible protein modifications involved in prokaryotic and eukaryotic transcriptional regulation.

McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR.,Fuhrmann J, Schmidt A, Spiess S, Lehner A, Turgay K, Mechtler K, Charpentier E, Clausen T Science. 2009 Jun 5;324(5932):1323-7. PMID:19498169[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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References

  1. Fuhrmann J, Schmidt A, Spiess S, Lehner A, Turgay K, Mechtler K, Charpentier E, Clausen T. McsB is a protein arginine kinase that phosphorylates and inhibits the heat-shock regulator CtsR. Science. 2009 Jun 5;324(5932):1323-7. PMID:19498169 doi:324/5932/1323

Contents


PDB ID 3h0d

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