First time at Proteopedia? Click on the green links: they change the 3D image. Click and drag the molecules. Proteopedia is a 3D, interactive encyclopedia of proteins, RNA, DNA and other molecules. With a free user account, you can edit pages in Proteopedia. Visit the Main Page to learn more.

3h4b

From Proteopedia

Jump to: navigation, search


3h4b, resolution 2.85Å ()
Ligands: ,
Non-Standard Residues: ,
Gene: POLI, RAD30B (Homo sapiens)
Activity: DNA-directed DNA polymerase, with EC number 2.7.7.7
Related: 3h40, 3h4o
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Contents

Ternary complex of human DNA polymerase iota with template U/T and incoming dATP

Publication Abstract from PubMed

Human DNA polymerase-iota (Poliota) incorporates correct nucleotides opposite template purines with a much higher efficiency and fidelity than opposite template pyrimidines. In fact, the fidelity opposite template T is so poor that Poliota inserts an incorrect dGTP approximately 10 times better than it inserts the correct dATP. We determine here how a template T/U is accommodated in the Poliota active site and why a G is incorporated more efficiently than an A. We show that in the absence of incoming dATP or dGTP (binary complex), template T/U exists in both syn and anti conformations, but in the presence of dATP or dGTP (ternary complexes), template T/U is predominantly in the anti conformation. We also show that dATP and dGTP insert differently opposite template T/U, and that the basis of selection of dGTP over dATP is a hydrogen bond between the N2 amino group of dGTP and Gln59 of Poliota.

Replication across template T/U by human DNA polymerase-iota., Jain R, Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK, Structure. 2009 Jul 15;17(7):974-80. PMID:19604477

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

About this Structure

3h4b is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

See Also

Reference

  • Jain R, Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK. Replication across template T/U by human DNA polymerase-iota. Structure. 2009 Jul 15;17(7):974-80. PMID:19604477 doi:S0969-2126(09)00216-0

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools