3kza
From Proteopedia
Crystal structure of Gyuba, a patched chimera of b-lactglobulin
Structural highlights
FunctionLACB_BOVIN Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.LACB1_HORSE Lactoglobulin is the primary component of whey, it binds retinol and is probably involved in the transport of that molecule. Publication Abstract from PubMedbeta-lactoglobulin (LG) contains nine beta-strands (strands A-I) and one alpha-helix. Strands A-H form a beta-barrel. At neutral pH, equine LG (ELG) is monomeric, whereas bovine LG (BLG) is dimeric, and the I-strands of its two subunits form an intermolecular beta-sheet. We previously constructed a chimeric ELG in which the sequence of the I-strand was replaced with that of BLG. This chimera did not dimerize. For this study, we constructed the new chimera we call Gyuba (which means cow and horse in Japanese). The amino acid sequence of Gyuba includes the sequences of the BLG secondary structures and those of the ELG loops. The crystal structure of Gyuba is very similar to that of BLG and indicates that Gyuba dimerizes via the intermolecular beta-sheet formed by the two I-strands. Thus, the entire arrangement of the secondary structural elements is important for LG dimer formation. Structure and stability of Gyuba, a beta-lactoglobulin chimera.,Ohtomo H, Konuma T, Utsunoiya H, Tsuge H, Ikeguchi M Protein Sci. 2011 Nov;20(11):1867-75. doi: 10.1002/pro.720. Epub 2011 Sep, 22. PMID:21853497[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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