4fix

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Crystal Structure of GlfT2

Structural highlights

4fix is a 2 chain structure with sequence from Mycobacterium tuberculosis H37Rv. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.45Å
Ligands:BME, GOL, MN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GLFT2_MYCTU Involved in the polymerization of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component the mycobacteria cell wall, through successively beta-D-(1->5) and beta-D-(1->6)-galactofuranosyltransferases activities. It transfers the galactofuranosyl (Galf) unit from UDP-galactofuranosyl (UDP-Galf) onto the galactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl (Galf-Rha-GlcNAc-PP-C55), yielding polygalactofuranosyl-rhamnosyl-GlcNAc-pyrophosphoryl-undecaprenyl ((Galf)x-Rha-GlcNAc-PP-C55). It is able to transfer Galf onto beta-D-(1->5) or beta-D-(1->6) linked acceptor, but has a stronger affinity for beta-D-(1->6) acceptor.[1] [2] [3] [4] [5] [6]

References

  1. ↑ Mikusova K, Yagi T, Stern R, McNeil MR, Besra GS, Crick DC, Brennan PJ. Biosynthesis of the galactan component of the mycobacterial cell wall. J Biol Chem. 2000 Oct 27;275(43):33890-7. PMID:10934214 doi:http://dx.doi.org/10.1074/jbc.M006875200
  2. ↑ Kremer L, Dover LG, Morehouse C, Hitchin P, Everett M, Morris HR, Dell A, Brennan PJ, McNeil MR, Flaherty C, Duncan K, Besra GS. Galactan biosynthesis in Mycobacterium tuberculosis. Identification of a bifunctional UDP-galactofuranosyltransferase. J Biol Chem. 2001 Jul 13;276(28):26430-40. Epub 2001 Apr 13. PMID:11304545 doi:http://dx.doi.org/10.1074/jbc.M102022200
  3. ↑ Rose NL, Completo GC, Lin SJ, McNeil M, Palcic MM, Lowary TL. Expression, purification, and characterization of a galactofuranosyltransferase involved in Mycobacterium tuberculosis arabinogalactan biosynthesis. J Am Chem Soc. 2006 May 24;128(20):6721-9. PMID:16704275 doi:http://dx.doi.org/10.1021/ja058254d
  4. ↑ Belanova M, Dianiskova P, Brennan PJ, Completo GC, Rose NL, Lowary TL, Mikusova K. Galactosyl transferases in mycobacterial cell wall synthesis. J Bacteriol. 2008 Feb;190(3):1141-5. Epub 2007 Nov 30. PMID:18055597 doi:http://dx.doi.org/10.1128/JB.01326-07
  5. ↑ Rose NL, Zheng RB, Pearcey J, Zhou R, Completo GC, Lowary TL. Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase. Carbohydr Res. 2008 Aug 11;343(12):2130-9. doi: 10.1016/j.carres.2008.03.023., Epub 2008 Mar 24. PMID:18423586 doi:http://dx.doi.org/10.1016/j.carres.2008.03.023
  6. ↑ May JF, Splain RA, Brotschi C, Kiessling LL. A tethering mechanism for length control in a processive carbohydrate polymerization. Proc Natl Acad Sci U S A. 2009 Jul 21;106(29):11851-6. doi:, 10.1073/pnas.0901407106. Epub 2009 Jul 1. PMID:19571009 doi:http://dx.doi.org/10.1073/pnas.0901407106

Contents


PDB ID 4fix

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