4nn0
From Proteopedia
Crystal structure of the C1QTNF5 globular domain in space group P63
Structural highlights
DiseaseC1QT5_HUMAN Late-onset retinal degeneration. The disease is caused by mutations affecting the gene represented in this entry. FunctionPublication Abstract from PubMedThe C1q-tumor necrosis factor 5 (C1QTNF5) protein plays a significant role in retinal pigmented epithelium (RPE) cellular adhesion. The C1QTNF5 gene is co-transcribed with the frizzled-related protein (MFRP) gene. A Ser-to-Arg mutation at site 163 (S163R) in C1QTNF5 is known to cause late-onset retinal macular degeneration (L-ORMD). Here we also found that C1QTNF5 monomers can multimerize into a bouquet-like octadecamer. We found that a novel intermolecular hydrogen-bond network of S163 that glues adjacent globular heads of C1QTNF5 together was weakened or abolished by the R163 pathogenic mutation. These findings could underlie the structural basis of this protein's adhesive function and relate to the pathogenesis of its S163R mutation. Additionally, the fact that C1QTNF5 immobilized to a resin selectively enriched detergent extracted membrane-bound MFRP, further confirmed their interaction, implying functions other than cellular adhesion for C1QTNF5. The macular degeneration-linked C1QTNF5 (S163) mutation causes higher-order structural rearrangements.,Tu X, Palczewski K J Struct Biol. 2014 Feb 12. pii: S1047-8477(14)00025-2. doi:, 10.1016/j.jsb.2014.02.001. PMID:24531000[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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