4qfw

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Crystal structure of acyl-CoA thioesterase tesB from Yersinia pestis

Structural highlights

4qfw is a 4 chain structure with sequence from Yersinia pestis. This structure supersedes the now removed PDB entry 4gwh. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A3N4BCC7_YERPE

Publication Abstract from PubMed

Yersinia pestis is a highly virulent human pathogen and is the causative agent of bubonic plague. Spread through the bite of infected fleas, plague epidemics have marked important events in history, including the Justinian plague (6th century), the Black Death (14th century) which decimated nearly one quarter of the European population, and more recently the Orientalis plague (1894). To date, deaths are still being reported and, without treatment, the disease kills most people within 4 days. One of the thioesterases from Y. pestis, TesB, is a broad-range acyl-CoA thioesterase and is highly conserved within prokaryotes and throughout evolution, sharing sequence similarity with the HIV Nef binding protein ACOT8. Here the expression, purification, crystallization and diffraction of TesB are reported. TesB has been recombinantly expressed and crystallized using the vapour-diffusion hanging-drop technique at pH 7.0 and 290 K. After optimization, crystals diffracted to 2.0 A resolution at the Australian Synchrotron and belong to the space group P12(1)1 (a = 73.55, b = 170.82, c = 101.98 A), with eight molecules likely to be present in the asymmetric unit.

Crystallization of the acyl-CoA thioesterase TesB from Yersinia pestis.,Swarbrick CM, Patterson EI, Forwood JK Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Feb 1;69(Pt 2):188-90., doi: 10.1107/S1744309113001267. Epub 2013 Jan 31. PMID:23385765[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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See Also

References

  1. Swarbrick CM, Patterson EI, Forwood JK. Crystallization of the acyl-CoA thioesterase TesB from Yersinia pestis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Feb 1;69(Pt 2):188-90., doi: 10.1107/S1744309113001267. Epub 2013 Jan 31. PMID:23385765 doi:http://dx.doi.org/10.1107/S1744309113001267

Contents


PDB ID 4qfw

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