4v5j

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Structure of the 70S ribosome bound to Release factor 2 and a substrate analog provides insights into catalysis of peptide release

Structural highlights

4v5j is a 20 chain structure with sequence from Thermus thermophilus HB8. This structure supersedes the now removed PDB entries 2x9r, 2x9s, 2x9t and 2x9u. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.1Å
Ligands:8AN, MG, PHA, ZN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RS2_THET8 Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B]

Publication Abstract from PubMed

We report the crystal structure of release factor 2 bound to ribosome with an aminoacyl tRNA substrate analog at the ribosomal P site, at 3.1 A resolution. The structure shows that upon stop-codon recognition, the universally conserved GGQ motif packs tightly into the peptidyl transferase center. Nucleotide A2602 of 23S rRNA, implicated in peptide release, packs with the GGQ motif in release factor 2. The ribose of A76 of the peptidyl-tRNA adopts the C2'-endo conformation, and the 2' hydroxyl of A76 is within hydrogen-bond distance of the 2' hydroxyl of A2451. The structure suggests how a catalytic water can be coordinated in the peptidyl transferase center and, together with previous biochemical and computational data, suggests a model for how the ester bond between the peptidyl tRNA and the nascent peptide is hydrolyzed.

Structure of the 70S ribosome bound to release factor 2 and a substrate analog provides insights into catalysis of peptide release.,Jin H, Kelley AC, Loakes D, Ramakrishnan V Proc Natl Acad Sci U S A. 2010 Apr 26. PMID:20421507[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
14 reviews cite this structure
The elongation, termination, and recycling phases of translation in eukaryotes.
Dever et al. (2012)
13 more
44 other articles
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See Also

References

  1. ↑ Jin H, Kelley AC, Loakes D, Ramakrishnan V. Structure of the 70S ribosome bound to release factor 2 and a substrate analog provides insights into catalysis of peptide release. Proc Natl Acad Sci U S A. 2010 Apr 26. PMID:20421507

Contents


PDB ID 4v5j

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