4w99
From Proteopedia
Apo-structure of the Y79F,W322E-double mutant of Etr1p
Structural highlights
FunctionETR1_CANTR Required for respiration and the maintenance of the mitochondrial compartment. May have a role in the mitochondrial synthesis of fatty acids.[1] Publication Abstract from PubMedAn improved understanding of enzymes' catalytic proficiency and stereoselectivity would further enable applications in chemistry, biocatalysis and industrial biotechnology. We use a chemical probe to dissect individual catalytic steps of enoyl-thioester reductases (Etrs), validating an active site tyrosine as the cryptic proton donor and explaining how it had eluded definitive identification. This information enabled the rational redesign of Etr, yielding mutants that create products with inverted stereochemistry at wild type-like turnover frequency. The use of ene adducts to study and engineer enoyl-thioester reductases.,Rosenthal RG, Vogeli B, Quade N, Capitani G, Kiefer P, Vorholt JA, Ebert MO, Erb TJ Nat Chem Biol. 2015 Apr 13. doi: 10.1038/nchembio.1794. PMID:25867044[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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