5eid

Structural highlights

Function

AKR2A_ARATH Exhibits chaperone activity toward chloroplast outer envelope membrane, mitochondrion outer membrane, endoplasmic reticulum membrane and peroxisomal proteins, by recruiting specific proteins containing a single transmembrane associated with an AKR2A-binding sequence (ABS) and subsequently binding glycolipids (e.g. monogalactosyldiacylglycerol (MGDG) and phosphatidylglycerol (PG)) present in the membrane of the target organelle (PubMed:18193034, PubMed:20215589, PubMed:25203210). Seems to be involved in the regulation of hydrogen peroxide levels during biotic and abiotic stresses by optimizing the ascorbate peroxidase 3 (APX3) hydrogen peroxide-degrading activity. This regulation might be monitored by GRF6. Cytosolic targeting factor for chloroplast outer membrane (COM) proteins that mediates sorting and targeting of nascent chloroplast outer envelope membrane (OEM) proteins to the chloroplast. Facilitates the targeting of OEP7 to chloroplasts (PubMed:18193034). Facilitates the targeting of APX3 to peroxisomes. Involved in cellular metabolism (e.g. peroxisome activity) and required for plant growth and development (PubMed:20215589).^{[1] [2] [3] [4]}

See Also

• Ankyrin repeat domain-containing protein 3D structures

References

1. ↑ Yan J, Wang J, Zhang H. An ankyrin repeat-containing protein plays a role in both disease resistance and antioxidation metabolism. Plant J. 2002 Jan;29(2):193-202. PMID:11862948 doi:10.1046/j.0960-7412.2001.01205.x
2. ↑ Bae W, Lee YJ, Kim DH, Lee J, Kim S, Sohn EJ, Hwang I. AKR2A-mediated import of chloroplast outer membrane proteins is essential for chloroplast biogenesis. Nat Cell Biol. 2008 Feb;10(2):220-7. PMID:18193034 doi:10.1038/ncb1683
3. ↑ Shen G, Kuppu S, Venkataramani S, Wang J, Yan J, Qiu X, Zhang H. ANKYRIN REPEAT-CONTAINING PROTEIN 2A is an essential molecular chaperone for peroxisomal membrane-bound ASCORBATE PEROXIDASE3 in Arabidopsis. Plant Cell. 2010 Mar;22(3):811-31. PMID:20215589 doi:10.1105/tpc.109.065979
4. ↑ Kim DH, Park MJ, Gwon GH, Silkov A, Xu ZY, Yang EC, Song S, Song K, Kim Y, Yoon HS, Honig B, Cho W, Cho Y, Hwang I. An Ankyrin Repeat Domain of AKR2 Drives Chloroplast Targeting through Coincident Binding of Two Chloroplast Lipids. Dev Cell. 2014 Sep 8;30(5):598-609. doi: 10.1016/j.devcel.2014.07.026. PMID:25203210 doi:http://dx.doi.org/10.1016/j.devcel.2014.07.026