5eo8

Structural highlights

Function

LECF_ASPOR Lectin that specifically binds to L-fucose and weakly reacts with mannose and N-acetyl-neuraminic acid (PubMed:12092808, PubMed:27318092, PubMed:28041800). Has strongest preference for the alpha-1,6-fucosylated chain (core fucose) on glycoproteins among alpha-1,2-, alpha-1,3-, alpha-1,4-, and alpha-1,6-fucosylated chains (PubMed:17383961, PubMed:19109923). Binds to fucose residues of IgE in mice and human, causing antigen-independent IgE-mediated mast cell activation and anaphylactoid reactions in mice and is possibly implicated in allergic response to Aspergillus oryzae in humans (PubMed:21790704). Induces secretion of pro-inflammatory cytokines IL6 and IL8 implicated in ocular diseases such as mycotic keratitis, probably through its interaction with host toll-like receptors TLR2 and TLR4, followed by up-regulation of pro-inflammatory cytokines (PubMed:28470344).^{[1] [2] [3] [4] [5] [6] [7]}

References

1. ↑ Ishida H, Moritani T, Hata Y, Kawato A, Suginami K, Abe Y, Imayasu S. Molecular cloning and overexpression of fleA gene encoding a fucose-specific lectin of Aspergillus oryzae. Biosci Biotechnol Biochem. 2002 May;66(5):1002-8. PMID:12092808 doi:10.1271/bbb.66.1002
2. ↑ Matsumura K, Higashida K, Ishida H, Hata Y, Yamamoto K, Shigeta M, Mizuno-Horikawa Y, Wang X, Miyoshi E, Gu J, Taniguchi N. Carbohydrate binding specificity of a fucose-specific lectin from Aspergillus oryzae: a novel probe for core fucose. J Biol Chem. 2007 May 25;282(21):15700-8. PMID:17383961 doi:10.1074/jbc.M701195200
3. ↑ Matsumura K, Higashida K, Hata Y, Kominami J, Nakamura-Tsuruta S, Hirabayashi J. Comparative analysis of oligosaccharide specificities of fucose-specific lectins from Aspergillus oryzae and Aleuria aurantia using frontal affinity chromatography. Anal Biochem. 2009 Mar 15;386(2):217-21. PMID:19109923 doi:10.1016/j.ab.2008.11.044
4. ↑ Yamaki K, Yoshino S. Aspergillus oryzae lectin induces anaphylactoid oedema and mast cell activation through its interaction with fucose of mast cell-bound non-specific IgE. Scand J Immunol. 2011 Nov;74(5):445-53. PMID:21790704 doi:10.1111/j.1365-3083.2011.02598.x
5. ↑ Makyio H, Shimabukuro J, Suzuki T, Imamura A, Ishida H, Kiso M, Ando H, Kato R. Six independent fucose-binding sites in the crystal structure of Aspergillus oryzae lectin. Biochem Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30987-1. doi:, 10.1016/j.bbrc.2016.06.069. PMID:27318092 doi:http://dx.doi.org/10.1016/j.bbrc.2016.06.069
6. ↑ Shimabukuro J, Makyio H, Suzuki T, Nishikawa Y, Kawasaki M, Imamura A, Ishida H, Ando H, Kato R, Kiso M. Synthesis of seleno-fucose compounds and their application to the X-ray structural determination of carbohydrate-lectin complexes using single/multi-wavelength anomalous dispersion phasing. Bioorg Med Chem. 2016 Dec 19. pii: S0968-0896(16)31172-5. doi:, 10.1016/j.bmc.2016.12.021. PMID:28041800 doi:http://dx.doi.org/10.1016/j.bmc.2016.12.021
7. ↑ Ballal S, Belur S, Laha P, Roy S, Swamy BM, Inamdar SR. Mitogenic lectins from Cephalosporium curvulum (CSL) and Aspergillus oryzae (AOL) mediate host-pathogen interactions leading to mycotic keratitis. Mol Cell Biochem. 2017 Oct;434(1-2):209-219. PMID:28470344 doi:10.1007/s11010-017-3050-9