5hk8

Structural highlights

Function

MES16_ARATH Involved in the chlorophyll breakdown by its action in fluorescent chlorophyll catabolites (FCCs) demethylation (PubMed:22147518, PubMed:23723324, PubMed:24302623). Demethylates the C13(2)-carboxymethyl group present at the isocyclic ring of chlorophyll (PubMed:22147518). Uses primary fluorescent dioxobilin-type chlorophyll catabolite (pFDCC) as substrate to produce O13(4)-desmethyl pFDCC (PubMed:23723324, PubMed:24302623). Also able to catalyze pheophorbides in vitro (PubMed:22147518). Methylesterase shown to have carboxylesterase activity, methyl indole-3-acetic acid (MeIAA) esterase activity and methyl jasmonate (MeJA) esterase activity in vitro (PubMed:18467465, PubMed:27109476).^{[1] [2] [3] [4] [5]}

References

1. ↑ Yang Y, Xu R, Ma CJ, Vlot AC, Klessig DF, Pichersky E. Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated by several esterases belonging to the AtMES esterase family of Arabidopsis. Plant Physiol. 2008 Jul;147(3):1034-45. doi: 10.1104/pp.108.118224. Epub 2008 May, 8. PMID:18467465 doi:http://dx.doi.org/10.1104/pp.108.118224
2. ↑ Christ B, Schelbert S, Aubry S, Sussenbacher I, Muller T, Krautler B, Hortensteiner S. MES16, a member of the methylesterase protein family, specifically demethylates fluorescent chlorophyll catabolites during chlorophyll breakdown in Arabidopsis. Plant Physiol. 2012 Feb;158(2):628-41. doi: 10.1104/pp.111.188870. Epub 2011 Dec , 6. PMID:22147518 doi:http://dx.doi.org/10.1104/pp.111.188870
3. ↑ Christ B, Süssenbacher I, Moser S, Bichsel N, Egert A, Müller T, Kräutler B, Hörtensteiner S. Cytochrome P450 CYP89A9 is involved in the formation of major chlorophyll catabolites during leaf senescence in Arabidopsis. Plant Cell. 2013 May;25(5):1868-80. PMID:23723324 doi:10.1105/tpc.113.112151
4. ↑ Süssenbacher I, Christ B, Hörtensteiner S, Kräutler B. Hydroxymethylated phyllobilins: a puzzling new feature of the dioxobilin branch of chlorophyll breakdown. Chemistry. 2014 Jan 3;20(1):87-92. PMID:24302623 doi:10.1002/chem.201303398
5. ↑ Li H, Pu H. Crystal structure of methylesterase family member 16 (MES16) from Arabidopsis thaliana. Biochem Biophys Res Commun. 2016 May 20;474(1):226-231. PMID:27109476 doi:10.1016/j.bbrc.2016.04.115