5jcv

Structural highlights

Function

SRTB_LISMO Transpeptidase that anchors surface proteins to the cell wall (Probable) (PubMed:16247833, PubMed:19129190). Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall (Probable). Catalyzes a cell wall sorting reaction in which a surface protein with the consensus sorting signal NP(Q/K)(T/S)(N/G/S)(D/A) is cleaved between the fourth and fifth residues, and the fourth position is linked to the cell wall (Probable) (PubMed:19129190). This is not the major sortase in Listeria, it seems to anchor only 2 proteins, Hbp2 (SvpA) and Hbp1 (PubMed:15028680, PubMed:16247833).^{[1] [2] [3] [4] [5]}

References

1. ↑ Bierne H, Garandeau C, Pucciarelli MG, Sabet C, Newton S, Garcia-del Portillo F, Cossart P, Charbit A. Sortase B, a new class of sortase in Listeria monocytogenes. J Bacteriol. 2004 Apr;186(7):1972-82. PMID:15028680 doi:10.1128/JB.186.7.1972-1982.2004
2. ↑ Pucciarelli MG, Calvo E, Sabet C, Bierne H, Cossart P, García-del Portillo F. Identification of substrates of the Listeria monocytogenes sortases A and B by a non-gel proteomic analysis. Proteomics. 2005 Dec;5(18):4808-17. PMID:16247833 doi:10.1002/pmic.200402075
3. ↑ Mariscotti JF, García-del Portillo F, Pucciarelli MG. The Listeria monocytogenes sortase-B recognizes varied amino acids at position 2 of the sorting motif. J Biol Chem. 2009 Mar 6;284(10):6140-6. PMID:19129190 doi:10.1074/jbc.M807989200
4. ↑ Bierne H, Garandeau C, Pucciarelli MG, Sabet C, Newton S, Garcia-del Portillo F, Cossart P, Charbit A. Sortase B, a new class of sortase in Listeria monocytogenes. J Bacteriol. 2004 Apr;186(7):1972-82. PMID:15028680 doi:10.1128/JB.186.7.1972-1982.2004
5. ↑ Pucciarelli MG, Calvo E, Sabet C, Bierne H, Cossart P, García-del Portillo F. Identification of substrates of the Listeria monocytogenes sortases A and B by a non-gel proteomic analysis. Proteomics. 2005 Dec;5(18):4808-17. PMID:16247833 doi:10.1002/pmic.200402075