5jql

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Crystal Structure of Phosphatidic acid Transporter Ups1/Mdm35 Void of Bound Phospholipid from Saccharomyces Cerevisiae at 2.9 Angstroms Resolution

Structural highlights

5jql is a 12 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.9Å
Ligands:MSE
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

UPS1_YEAST Required for maintenance of normal mitochondrial morphology as well as PCP1-dependent processing of MGM1. With UPS2, controls the level of cardiolipin in mitochondria. Cardiolipin is a unique phospholipid with four fatty acid chains and is present mainly in the mitochondrial inner membrane where it stabilizes the electron transport chain supercomplex between complexes III and IV through direct interaction of their subunits.[1] [2] [3] [4]

Publication Abstract from PubMed

Cardiolipin, an essential mitochondrial physiological regulator, is synthesized from phosphatidic acid (PA) in the inner mitochondrial membrane (IMM). PA is synthesized in the endoplasmic reticulum and transferred to the IMM via the outer mitochondrial membrane (OMM) under mediation by the Ups1/Mdm35 protein family. Despite the availability of numerous crystal structures, the detailed mechanism underlying PA transfer between mitochondrial membranes remains unclear. Here, a model of Ups1/Mdm35-membrane interaction is established using combined crystallographic data, all-atom molecular dynamics simulations, extensive structural comparisons, and biophysical assays. The alpha2-loop, L2-loop, and alpha3 helix of Ups1 mediate membrane interactions. Moreover, non-complexed Ups1 on membranes is found to be a key transition state for PA transfer. The membrane-bound non-complexed Ups1/ membrane-bound Ups1 ratio, which can be regulated by environmental pH, is inversely correlated with the PA transfer activity of Ups1/Mdm35. These results demonstrate a new model of the fine conformational changes of Ups1/Mdm35 during PA transfer.

Molecular mechanism of mitochondrial phosphatidate transfer by Ups1.,Lu J, Chan C, Yu L, Fan J, Sun F, Zhai Y Commun Biol. 2020 Aug 25;3(1):468. doi: 10.1038/s42003-020-01121-x. PMID:32843686[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
3 reviews cite this structure
Mechanisms of Non-Vesicular Exchange of Lipids at Membrane Contact Sites: Of Shuttles, Tunnels and, Funnels.
Egea et al. (2021)
2 more
2 other articles
No citations found

References

  1. ↑ Sesaki H, Dunn CD, Iijima M, Shepard KA, Yaffe MP, Machamer CE, Jensen RE. Ups1p, a conserved intermembrane space protein, regulates mitochondrial shape and alternative topogenesis of Mgm1p. J Cell Biol. 2006 Jun 5;173(5):651-8. PMID:16754953 doi:http://dx.doi.org/10.1083/jcb.200603092
  2. ↑ Osman C, Haag M, Potting C, Rodenfels J, Dip PV, Wieland FT, Brugger B, Westermann B, Langer T. The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria. J Cell Biol. 2009 Feb 23;184(4):583-96. doi: 10.1083/jcb.200810189. Epub 2009 Feb, 16. PMID:19221197 doi:http://dx.doi.org/10.1083/jcb.200810189
  3. ↑ Tamura Y, Endo T, Iijima M, Sesaki H. Ups1p and Ups2p antagonistically regulate cardiolipin metabolism in mitochondria. J Cell Biol. 2009 Jun 15;185(6):1029-45. Epub 2009 Jun 8. PMID:19506038 doi:http://dx.doi.org/jcb.200812018
  4. ↑ Tamura Y, Iijima M, Sesaki H. Mdm35p imports Ups proteins into the mitochondrial intermembrane space by functional complex formation. EMBO J. 2010 Sep 1;29(17):2875-87. doi: 10.1038/emboj.2010.149. Epub 2010 Jul 9. PMID:20622808 doi:http://dx.doi.org/10.1038/emboj.2010.149
  5. ↑ Lu J, Chan C, Yu L, Fan J, Sun F, Zhai Y. Molecular mechanism of mitochondrial phosphatidate transfer by Ups1. Commun Biol. 2020 Aug 25;3(1):468. doi: 10.1038/s42003-020-01121-x. PMID:32843686 doi:http://dx.doi.org/10.1038/s42003-020-01121-x

Contents


PDB ID 5jql

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