5lis

From Proteopedia

Insulin solved by Native SAD from a dataset collected in one second

PDB ID 5lis

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Structural highlights

5lis is a 2 chain structure with sequence from Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.293Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

INS_PIG Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Publication Abstract from PubMed

The development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. The new EIGER detector features a pixel size of 75 x 75 microm, frame rates of up to 3000 Hz and a dead time as low as 3.8 micros. An EIGER 1M and EIGER 16M were tested on Swiss Light Source beamlines X10SA and X06SA for their application in macromolecular crystallography. The combination of fast frame rates and a very short dead time allows high-quality data acquisition in a shorter time. The ultrafine varphi-slicing data-collection method is introduced and validated and its application in finding the optimal rotation angle, a suitable rotation speed and a sufficient X-ray dose are presented. An improvement of the data quality up to slicing at one tenth of the mosaicity has been observed, which is much finer than expected based on previous findings. The influence of key data-collection parameters on data quality is discussed.

EIGER detector: application in macromolecular crystallography.,Casanas A, Warshamanage R, Finke AD, Panepucci E, Olieric V, Noll A, Tampe R, Brandstetter S, Forster A, Mueller M, Schulze-Briese C, Bunk O, Wang M Acta Crystallogr D Struct Biol. 2016 Sep;72(Pt 9):1036-48. doi:, 10.1107/S2059798316012304. Epub 2016 Aug 31. PMID:27599736^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Casanas A, Warshamanage R, Finke AD, Panepucci E, Olieric V, Noll A, Tampe R, Brandstetter S, Forster A, Mueller M, Schulze-Briese C, Bunk O, Wang M. EIGER detector: application in macromolecular crystallography. Acta Crystallogr D Struct Biol. 2016 Sep;72(Pt 9):1036-48. doi:, 10.1107/S2059798316012304. Epub 2016 Aug 31. PMID:27599736 doi:http://dx.doi.org/10.1107/S2059798316012304