5o60
From Proteopedia
Structure of the 50S large ribosomal subunit from Mycobacterium smegmatis
Structural highlights
FunctionPublication Abstract from PubMedThe ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. Here, we present the 3.3-A cryo-EM structure of the 70S ribosome of Mycobacterium smegmatis, a close relative to the human pathogen Mycobacterium tuberculosis. The structure reveals two additional ribosomal proteins and localizes them to the vicinity of drug-target sites in both the catalytic center and the decoding site of the ribosome. Furthermore, we visualized actinobacterium-specific rRNA and protein expansions that extensively remodel the ribosomal surface with implications for polysome organization. Our results provide a foundation for understanding the idiosyncrasies of mycobacterial translation and reveal atomic details of the structure that will facilitate the design of anti-tubercular therapeutics. The Complete Structure of the Mycobacterium smegmatis 70S Ribosome.,Hentschel J, Burnside C, Mignot I, Leibundgut M, Boehringer D, Ban N Cell Rep. 2017 Jul 5;20(1):149-160. doi: 10.1016/j.celrep.2017.06.029. PMID:28683309[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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