7lmb

Structural highlights

Function

LARP7_TETTS RNA-binding protein required for assembly of the holoenzyme telomerase ribonucleoprotein (RNP) complex (PubMed:15131081, PubMed:15696174, PubMed:16507983, PubMed:17322903, PubMed:20713447, PubMed:22315458, PubMed:22705372). Telomerase is an essential ribonucleoprotein enzyme that copies new telomeric repeats onto chromosome ends by repetitively synthesizing the short telomere-repeat sequence 5'-TTGGGG-3' using an RNA template component TER (PubMed:15696174, PubMed:17322903, PubMed:20713447). TAP65/p65 specifically binds telomerase RNA template TER and is required for biogenesis and placement of the TER stem-terminus element: TAP65/p65 first protects the 3'-end of TER from degradation and acts as a chaperone to correctly fold TER for protein binding; it then bends TER stem-loop IV to position it for interaction of stem-loop IV with catalytic TERT RNA-binding domain (PubMed:15696174, PubMed:17322903, PubMed:20713447, PubMed:22315458, PubMed:22705372).^{[1] [2] [3] [4] [5] [6] [7]}

See Also

• Telomerase 3D structures
• Telomerase-associated protein

References

1. ↑ Witkin KL, Collins K. Holoenzyme proteins required for the physiological assembly and activity of telomerase. Genes Dev. 2004 May 15;18(10):1107-18. doi: 10.1101/gad.1201704. Epub 2004 May 6. PMID:15131081 doi:http://dx.doi.org/10.1101/gad.1201704
2. ↑ Prathapam R, Witkin KL, O'Connor CM, Collins K. A telomerase holoenzyme protein enhances telomerase RNA assembly with telomerase reverse transcriptase. Nat Struct Mol Biol. 2005 Mar;12(3):252-7. doi: 10.1038/nsmb900. Epub 2005 Feb 6. PMID:15696174 doi:http://dx.doi.org/10.1038/nsmb900
3. ↑ O'Connor CM, Collins K. A novel RNA binding domain in tetrahymena telomerase p65 initiates hierarchical assembly of telomerase holoenzyme. Mol Cell Biol. 2006 Mar;26(6):2029-36. doi: 10.1128/MCB.26.6.2029-2036.2006. PMID:16507983 doi:http://dx.doi.org/10.1128/MCB.26.6.2029-2036.2006
4. ↑ Stone MD, Mihalusova M, O'connor CM, Prathapam R, Collins K, Zhuang X. Stepwise protein-mediated RNA folding directs assembly of telomerase ribonucleoprotein. Nature. 2007 Mar 22;446(7134):458-61. doi: 10.1038/nature05600. Epub 2007 Feb 25. PMID:17322903 doi:http://dx.doi.org/10.1038/nature05600
5. ↑ Berman AJ, Gooding AR, Cech TR. Tetrahymena telomerase protein p65 induces conformational changes throughout telomerase RNA (TER) and rescues telomerase reverse transcriptase and TER assembly mutants. Mol Cell Biol. 2010 Oct;30(20):4965-76. doi: 10.1128/MCB.00827-10. Epub 2010 Aug , 16. PMID:20713447 doi:http://dx.doi.org/10.1128/MCB.00827-10
6. ↑ Akiyama BM, Loper J, Najarro K, Stone MD. The C-terminal domain of Tetrahymena thermophila telomerase holoenzyme protein p65 induces multiple structural changes in telomerase RNA. RNA. 2012 Apr;18(4):653-60. doi: 10.1261/rna.031377.111. Epub 2012 Feb 7. PMID:22315458 doi:http://dx.doi.org/10.1261/rna.031377.111
7. ↑ Singh M, Wang Z, Koo BK, Patel A, Cascio D, Collins K, Feigon J. Structural Basis for Telomerase RNA Recognition and RNP Assembly by the Holoenzyme La Family Protein p65. Mol Cell. 2012 Jun 14. PMID:22705372 doi:10.1016/j.molcel.2012.05.018